Anti-Alpha-Synuclein SNCA Antibody

Product Name

Anti-Alpha-Synuclein SNCA Antibody

View all alpha-Synuclein Antibodies

SKU/Catalog Number

M00215-3

Size

100ul

Description

Boster Bio Anti-Alpha-Synuclein SNCA Antibody catalog # M00215-3. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Cow, Human, Mouse, Pig, Rat, Horse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alpha-Synuclein SNCA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00215-3)

Host

Chicken

Contents

Affinity purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN₃

Clonality

Polyclonal

Immunogen

Full length recombinant human protein expressed in and purified from E. coli

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00215-3 is reactive to SNCA in Cow, Human, Mouse, Pig, Rat, Horse

Applications

M00215-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

14.46kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2,000 IF/ICC and IHC: 1:1,000

Validation Images & Assay Conditions

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Western blot analysis of different tissue lysates using chicken pAb to -synuclein, M00215-3, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 15kDa corresponds to the -synuclein protein in brain extracts, which are rich in synapses, while a weaker band is seen in spinal cord extracts where synapses are a more minor component.

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Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to -synuclein, M00215-3, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45M, and free-floating sections were stained with above antibodies. The -synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.

Gene/Protein Information For SNCA (Source: Uniprot.org, NCBI)

Alternative Names

alpha-Synuclein; Lewy body) 4; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; non-A4 component of amyloid; PARK1; PARK4; SNCA; synuclein, alpha (non A4 component of amyloid precursor); Synuclein-alpha SNCA NACP, PARK1, PARK4, PD1 synuclein alpha alpha-synuclein|I+/--synuclein|non A-beta component of AD amyloid|synuclein alpha-140|synuclein, alpha (non A4 component of amyloid precursor)|truncated alpha synuclein

For more info on SNCA, check out the SNCA Infographic

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In this infographic, you will see the following information for SNCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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